ICAR NET Syllabus 2014-15 (ARS
Exam pattern) PDF Download
Agriculture Scientists Recruitment Board (ICAR, Ministry
of Agriculture, Govt. of India) separately conducts the examination for NET and
ARS. NET Exam is organized two times in a year while ARS Exam is organized only
once in a year. However, the course contents of both the examinations are same.
Get more update on recruitmentinfosite.com .Here we have provided the detailed
course content/syllabus of one discipline out of 55 disciplines. Candidates of
the followed mentioned discipline should take a look on the topics being asked
in the NET/ARS Exam while other disciplines’ candidates may download the PDF
attached at last.
Pattern of ARS (Agricultural
Research Services)
Preliminary Exam: 150 Marks (Objective Type Questions) in 2 hrs
duration (Negative Marking: 1/3rd)
Mains Exam: 240 Marks (Descriptive Type Questions) in 3
hrs duration
Viva Voce: 60 Marks
Pattern of NET Exam:
Paper 1: 150 Marks (Objective Type Questions) in 2
hrs duration (Negative Marking: 1/3rd)
Syllabus of NET/ARS Exam (Common
Syllabus):
01. AGRICULTURAL BIOTECHNOLOGY
Unit 1: Cell Structure and
Function
Prokaryotic and eukaryotic cell architecture, Cell wall,
plasma membrane, Structure and function of cell organelles: vacuoles,
mitochondria, plastids, golgi apparatus, ER, peroxisomes, glyoxisomes. Cell
division, regulation of cell cycle, Protein secretion and targeting, Cell
division, growth and differentiation.
Unit 2: Biomolecules and
Metabolism
Structure and function of carbohydrates, lipids, proteins
and nucleic acids, Synthesis of carbohydrate, glycolysis, HMP, Citric acid
cycle and metabolic regulation,Oxidative phosphorylation and substrate level
phosphorylation, Vitamins, plant and animal hormones. Functional molecules,
antioxidants, nutrient precursor, HSPs, anti-viral compounds.
Unit 3: Enzymology
Enzymes, structure conformation, classification, assay,
isolation, purification and characterization, catariysis specificity, mechanism
of action, active site, regulation of enzyme activity, multienzyme complexes,
immobilized enzymes and protein
engineering, immobilized enzymes and their application.
Unit 4: Molecular Genetics
Concept of gene, Prokaryotes as genetic system,
Prokaryotic and eukaryotic chromosomes, methods of gene isolation and
identification, Split genes, overlapping genes and pseudo genes, Organization
of prokaryotic and eukaryotic genes and genomes including operan, exon, intron,
enhancer promoter sequences and other regulatory elements. Mutation –
spontaneous, induced and site-directed, recombination in bacteria, fungi and
viruses, transformation, transduction, conjugation, transposable elements and
transposition.
Unit 5: Gene Expression
Expression of genetic information, operon concept,
Transcription – mechanism of transcription in prokaryotes and eukaryotes,
transcription unit, regulatory sequences and enhancers, activators, repressors,
co-activators, Co-repressors in prokaryotes and eukaryotes, inducible genes and
promoters, Transcription factors post transcriptional modification and protein
transport, DNA-protein interaction, Genetic code. Mechanism of translation and
its control, post translational modifications.
Unit 6: Molecular Biology Techniques
Isolation and purification of nucleic acids. Nucleic
acids hybridization: Southern, northern and western blotting hybridization.
Immune response monoclonal and polyclonal antibodies and ELISA, DNA sequencing.
Construction and screening of genomic and C-DNA libraries. Gel electrophoretic
techniques. Polymerase chain reactor spectroscopy, rtPCR ultracentrifugation,
chromatography, FISH, RIA etc.
Unit 7: Gene Cloning
Restriction enzymes and their uses. Salient features and
uses of most commonly used vectors i.e. plasmids, bacteriophages, phagmids,
cosmids, BACs, PACs and YACs, binary vectors, expression vectors. Gene cloning
and sub-cloning strategies, chromosome walking, genetic transformation, Basis
of animal cloning. Biology. Risk assessment and IPR.
Unit 8: Molecular Biology
Ribosome structure and function. Protein biosynthesis in
prokaryotes and ekaryotes. Post-translational modification. Gene regulation,
RNA processing and Post transcriptional modifications. Bioprospecting, biofortification,
gene pryrimiding and gene fusion, nbozyme technology.
Unit 9: Plant Molecular Biology
Photoregulation and phytochrome regulation of nuclear and
chloroplastic gene expression. Molecular mechanism of nitrogen fixation.
Molecular biology of various stresses, viz. abiotic stresses like drought,
salt, heavy metals and temperature; and biotic stresses like bacterial, fungal
and viral diseases. Signal transduction and its molecular basis, molecular
mechanism of plant hormone action mitochondrial control of fertility,
structure, organization and regulation of nuclear gene concerning storage
proteins and starch synthesis.
Unit 10: Tissue Culture
Basic techniques in cell culture and somatic cell
genetics. Regulation of cell cycle and cell division. Clonal propagation.
Concept of cellular totipotency. Anther culture, somaclonal and gametoclonal
variations. Hybrid embryo culture and embryo rescue, somatic hybridization and
cybridization. Application of tissue culture in crop improvement. Secondary
metabolite production. In vitro, mutagenesis, cryopreservation and plant tissue
culture repository.
Unit 11: Plant Genetic
Engineering
Isolation of genes of economic importance. Gene constructs
for tissue-specific expression. Different methods of gene transfer to plants, Potential applications of plant genetic engineering for
crop improvement, i.e. insect-pest resistance (insect, viral, fungal and bacterial
disease resistance), abiotic stress resistance, herbicide resistance, storage protein
quality, increasing shelf-life, oil quality, Current status of transgenics,
biosafty norms and controlled field trials and release of transgenics (GMOs).
Unit 12: Molecular Markers and
Genomics
DNA molecular markers: Principles, type and applications;
restriction fragment length polymorphism (RFLP), amplified fragment length
polymorphism (AFLP), randomly amplified polymorphic DNA sequences (RAPD),
Simple sequence repeats (SSR), Single nucleotide polymorphism (SNP), Structural
and functional genomics, gene mapping, genome mapping, gene tagging and
comparative genomics and application of genomics.
Official Website of ICAR: icar.org.in
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